Molecular weights from equilibrium sedimentation in a density gradient
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چکیده
منابع مشابه
Molecular weights of homogeneous coliphage DNA's from density-gradient sedimentation equilibrium.
The problem of obtaining meaningful molecular weights for high molecular weight DNA samples by sedimentation equilibrium in a density gradient has been solved by correcting the theory for thermodynamio non-ideality. Several different methods of virial corrections are suggested and examined to determine the best method of obtaining molecular weights at infinite dilution. A pronounced virial effe...
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In 1957, Meselson, Stahl, and Vinograd 1 introduced the remarkable technique of sedimentation equilibriuln in a density gradient for the study of the properties of high molecular weight DNA in solution. Because of the very high molecular weights of DNA, conventional sedimentation equilibrium cannot be used; for even at the lowest centrifugal fields which are practical, the force on these molecu...
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Sedimentation equilibrium in a density gradient has recently been developed into a reliable method for the determination of molecular weights of homogeneous DNA samples. The procedure and calibrations required for this method are outlined in this paper. In 1957, Meselson, Stahl and Vinograd' introduced a remarkable analytical technique for studying the properties of DNA and viruses. Sedimentati...
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Regulated molecular interactions are essential for cellular function and viability, and both homo- and hetero-interactions between all types of biomolecules play important cellular roles. This chapter focuses on interactions between membrane proteins. Knowing both the stoichiometries and stabilities of these interactions in hydrophobic environments is a prerequisite for understanding how this c...
متن کاملSedimentation equilibrium of DNA samples heterogeneous in density.
The problem of determining the molecular weight of DNA samples by sedimentation equilibrium in a buoyantcdensity gradient is considered for the case of DNA samples with density heterogeneity. By determining apparent molecular weights in two or more buoyant mediums, quantitative measure of the amount of density heterogeneity can be determined. This method may be employed to determine both the tr...
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ژورنال
عنوان ژورنال: Biopolymers
سال: 1967
ISSN: 0006-3525,1097-0282
DOI: 10.1002/bip.1967.360050709